Manchester United midfielder Marouane Fellaini withdrew from the Belgium squad on Tuesday with a calf injury.
Fellaini was injured on Sunday as United beat Swansea City 3-1, and he will not be able to feature for Belgium against Netherlands and Estonia.
Belgium manager Roberto Martinez said will not call up a replacement.
"Fellaini picked up an injury against Swansea City and will go back to his club," Martinez said in a news conference. "We have a core of 24 players, which is enough.
"Apart from Fellaini, everyone is in order. Mousa Dembele is back but will still be resting against Netherlands. We are counting on him against Estonia."
Martinez also said Manchester City's Kevin De Bruyne will play in Wednesday's friendly in Amsterdam dealing with fatigue.
"Kevin De Bruyne is feeling a little tired, but he will certainly appear in my selection Wednesday," Martinez said.