England players who take part in this summer's Euro 2012 tournament in Poland and Ukraine will not be selected to play for Great Britain at the London 2012 Olympics.
Team GB manager and England Under-21 coach Stuart Pearce, who had not initially ruled anyone out, described it as "a sensible football decision".
Pearce said: "We have had discussions as we come now to notifying the long list of the players who could potentially be selected and it is important to make clear what we have agreed regarding England players who will be at Euro 2012.
"We have agreed that any player who boards the plane to the European Championship in Ukraine and Poland will not be considered for selection to Team GB.
"It's a sensible football decision when you consider the demands on players, and it now leaves no room for doubt for players and their clubs about next summer and the two major tournaments.''
The FA this week began notifying home associations and players of those on a long list for potential selection. Players are asked to confirm whether they would be available.
Pearce's announcement will please club managers such as Arsenal's Arsene Wenger and Manchester United's Sir Alex Ferguson who have said it would be too much for players to play in both tournaments.
It has also been revealed the Olympic Stadium could have retractable seating installed over the running track after the London Games to make it more attractive to football fans.
West Ham, who are favourites to be chosen as the tenants, are looking at having the retractable seating as part of their new bid for the stadium.