Tottenham have applied to the High Court for a judicial review into Newham Council's planned funding for the Olympic Stadium after the 2012 Games.
Spurs failed in their attempts to win bidding rights for the stadium in February, after the Olympic Park Legacy Committee (OPLC) chose West Ham and Newham Council as preferred bidders.
Unhappy with the decision, Spurs immediately signalled their intent to launch a judicial review - writing letters to the OPLC, the Government and Newham Council.
And Spurs have now confirmed that they plan to press ahead, with a club statement reading: "The club has today sought permission from the High Court to bring a claim against the London Borough of Newham ("Newham") for judicial review of Newham's process in providing a loan for the conversion of the Olympic Stadium after the 2012 Games.
"The club wrote to Newham asking it to explain its reasons and justification for its decision, but Newham has declined to respond to this request for information. Due to the time limits which apply to claims for judicial review, the Club has had no alternative but to issue these proceedings in order to protect its position.
"The club continues to hold discussions with both local and national government bodies in order to seek to agree a feasible stadium solution."
The battle for the Stratford site was fiercely contested between Tottenham and West Ham, and the decision to give preferred bidding rights to the East Londoners caused much consternation at White Hart Lane.
However, after Spurs' initially stated their intention to force a judicial review, an OPLC spokeswoman said: "The Olympic Park Legacy Company ran a very rigorous and transparent process in its selection of the recommended preferred bidder. We have been supported by independent experts in their field in terms of legal, financial, commercial and technical advice."
The spokeswoman also indicated that should Tottenham's attempts to secure a judicial review prove successful, the OPLC would be happy to co-operate.
"We have been consistent, fair, objective and entirely equal in our dealings with the bidders from start to finish," she said. "We are confident that if these judicial review proceedings are pursued, our approach will be entirely vindicated by the courts."