Phil Bardsley is the latest Scotland player to pull out of the squad ahead of this weekend's friendly against Brazil.
The Sunderland defender has been forced to withdraw with a knee injury, his club have confirmed.
Scotland assistant manager Peter Houston revealed yesterday that Bardsley would be assessed by medical staff after suffering a knock at the weekend as the squad jetted to La Manga for a training camp ahead of Sunday's game.
The Salford-born full-back, who qualifies for Scotland through his father, has been capped three times.
A statement on Sunderland's website confirmed: ''Defender Phil Bardsley will miss Sunday's international game between Scotland and Brazil after picking up a knock in Sunderland's defeat against Liverpool.
''The full back was in line to pick up his fourth cap for Scotland, however, he has been withdrawn from the squad after suffering a knee injury.
''Bardsley will receive treatment and be monitored by the club's medical team over the coming days.''
Goalkeeper Iain Turner and Rangers midfielder Steven Naismith have also withdrawn from the squad ahead of the clash at the Emirates Stadium.