Liverpool have replaced reserve team coach John McMahon with academy technical director Pep Segura, who will take over on a temporary basis until the end of the season.
The club say the move is part of their planning for the future, with a new structure to be put in place in the summer.
Segura, who has previously coached the likes of Andres Iniesta and Cesc Fabregas through Barcelona's youth set-up, will be assisted by under-16s coach Steve Cooper for the reserves' remaining matches.
"The club have conducted a thorough review of all operations based at our academy as part of our overall strategy to constantly improve our youth and professional player performance," a club spokesman said.
"We have a clear vision of the way ahead and a number of changes will now be put in place to help us to build on the considerable progress already made.
"Pep Segura will step in as reserve team manager on a temporary basis for the remainder of this term only, before a revised structure is put in place this summer.
"He will be assisted over the next few months by Steve Cooper."
The reserves saw their ten-match unbeaten run come to an end in a 2-1 defeat at Blackpool last week.