Rangers have failed with a last-gasp appeal to the Scottish Football Association over Kevin Thomson's red card in Sunday's League Cup final.
The Ibrox club waited until moments before the 1700 GMT deadline to contest the dismissal in a calculated attempt to ensure the case could not be dealt with before Wednesday night's Scottish Cup quarter-final replay at Dundee United, freeing the midfielder to play.
However, it is understood the SFA were braced for such tactics and had match referee Craig Thomson poised to review video footage of the incident.
The official was satisfied with his decision to send off Thomson for a second-half lunge on St Mirren's Steven Thomson, so the Rangers star's suspension will begin immediately.
Had the referee admitted making a mistake, the matter would have been refereed to the SFA's review panel, meaning Thomson would have been available to play on Wednesday night.
The SFA said in a statement: "In accordance with the provisions for claims of wrongful dismissal, the referee, Craig Thomson, was requested to review the circumstances of Kevin Thomson's sending off for serious foul play against St Mirren in the Co-operative Insurance Cup Final match on Sunday, 21st March.
"The referee has indicated that he is content with his decision. Accordingly, the claim is dismissed automatically and will not be referred to the review panel.
"The decision of the referee to send off Kevin Thomson is confirmed and the player's automatic one-match suspension is applied, with 12 penalty points being added to his SPL disciplinary record. Furthermore, the player incurs a one-match fixed suspension from 4th April for reaching the disciplinary threshold of 18 points.''