A calamitous error by Sheffield United goalkeeper Ian Bennett handed Port Vale a shock Carling Cup first round win at Bramall Lane.
When dealing with an innocuous free-kick from Marc Richards, the United stopper inexplicably let the ball through his hands and could only watch as it trickled over the line to give Richards his second and Vale a famous victory.
Billy Sharp's neat finish had earlier cancelled out Richards' 20th-minute opener and despite dominating large swathes of the match, Sharp's effort was the only time the hosts could beat an inspired Chris Martin in the Vale goal.
Then the decisive moment of game came on the hour mark when Richards curled a tame 20-yard free-kick straight down the throat of Bennett, but the former Birmingham man spilled the ball over the line in front of a rapturous Vale following.
United rallied, forcing a fine double save from Martin who kept out close-range efforts from Jordan Robertson.
Grant Holt scored a hat-trick as Norwich responded to the nightmare of their 7-1 home defeat by Colchester on Saturday by claiming an emphatic 4-0 win at Yeovil.
The game was goalless at half-time but the Canaries, relegated from the Coca-Cola Championship last season, took the lead 10 minutes after half-time when Wes Hoolahan converted a penalty after Scott Murray fouled Cody McDonald.
Grant Holt doubled the lead with a header before two goals in eight minutes - the second from 25 yards - sealed the crushing victory.
Southampton, relegated alongside Norwich, moved into the second round following a 2-0 win over Northampton.
The Saints went ahead when Rickie Lambert scored his first goal for the club with a first-half header.
Adam Lallana then scored from close range in the 69th minute to seal victory.
Colchester could not replicate their form from the weekend and went out 2-1 at the first hurdle to Leyton Orient despite pulling back a last-minute goal.
The visitors got off to an ideal start when Adrian Patulea headed them ahead in the second minute with John Melligan doubling the lead in the 63rd minute.
The Us were reduced to 10 men when Clive Platt was dismissed for violent conduct following Ryan Jarvis' foul on Kemal Izzet.
The hosts scored from Simon Hackney's resulting free-kick but it was not enough.
Last season's losing play-off finalists Millwall had an emphatic 4-0 victory over League Two Bournemouth thanks to a hat-trick by Neil Harris following Gary Alexander's opener.
MK Dons were on the wrong end of a 4-1 home defeat by Swindon.
Jon-Paul McGovern put the visitors ahead with a 30-yard shot in the 41st minute before Jermaine Easter levelled with a penalty eight minutes after half time.
Billy Paynter restored the lead with the first of his two goals before David McCracken's headed own goal sealed a miserable night for the Dons.
A last-minute goal by Paul Mullin saw League Two Accrington Stanley topple Walsall 2-1, who had to play for 55 minutes with 10 men.
The League One side went ahead through Alex Nicholl's early right-footed shot but were then reduced to 10 men 10 minutes before half-time when Rhys Weston got his second yellow card.
Robert Grant (53) levelled before Mullins' late intervention.
A goal two minutes from time gave Southend a 2-1 win at Cheltenham.
Elvis Hammond gave the hosts the lead on the stroke of half time before Lee Barnard equalised with 13 minutes left and the same player headed the Shrimpers to a late victory.
A last-minute header from Scott Dobie earned Carlisle a win over Oldham, Darryl Duffy scored twice - and missed a penalty - as Bristol Rovers beat Aldershot 2-1 and Huddersfield beat Stockport 3-1 thanks to a Jordan Rhodes double and a Theo Robinson strike.
Andy Gray missed a penalty as Charlton went down 1-0 to Hereford.
The Addicks, who were relegated with Norwich and Southampton last season, had the chance to take the lead with the match an hour old but Gary's powerful penalty was saved by Adam Bartlett.
The match was goalless after 90 minutes and eight minutes into extra-time it was the League Two side who went ahead through Jonny Godsmark's close-range shot.