Alvaro Navarro's injury-time winner helped Defensor Sporting defeat Colombia's Independiente Medellin 4-3 to assure the Uruguayans of their place in the Copa Libertadores last 16.
Navarro had put the hosts ahead after 14 minutes before Luis Arias' equaliser. Diego Vera fired Jorge Da Silva's side back in front before the break only for Andres Ortiz to level again.
Diego De Souza put Defensor ahead for a third time to set up a thrilling climax as the Colombians equalised through Diego Cabrera's header early in injury-time.
But four minutes into added time Navarro scored a dramatic winner to end Independiente's premature celebrations.
The win sees the Montevideo-based team finish second in Group Four with eight points, one ahead of Independiente and five behind table-toppers Sao Paulo.
The Brazilians ended their group campaign with a 2-1 win over already-eliminated America of Cali.
Wilmer Parra had put the visitors ahead but a brace by Dagoberto gave the three points to Muricy Ramalho's side.
Another Brazilian side that ended top of their pool with 13 points were Cruzeiro, who defeated Ecuador's Deportivo Quito 2-0 in Belo Horizonte.
A header by Leo Fortunato and Luis Garcia's own-goal assured the victory for the 'Azulao', who ended the match with 10 players as Fabricio was dismissed as well as Deportivo Quito duo Mauricio Donoso and Tilson Minda.
Adilson Baptista's side ended with a five-point advantage over second placed Estudiantes of Argentina in Group Five.
The La Plata-based team managed a goalless draw in their visit to Bolivia's Universitario Sucre.
The Argentinians also booked their place in the round of 16 and coach Alejandro Sabella showed his confidence: "We gave our best and surpassed a very hard group.
"The real Copa Libertadores start from now on."
Finally, Brazil's Sport came back from being one goal behind to defeat Chileans Colo Colo 2-1 in Recife to take the outright lead in Group One.
After a goalless first half Rodrigo Millar sent the visitors ahead, but Moacir and Vandinho gave Nelsinho Baptista three vital points.
Sport therefore assured their berth in the next stage with 10 points at the top of the standings, three more than Colo Colo and compatriots Palmeiras.
Both Colo Colo and Palmeiras will clash in Santiago in next week's decisive round, while Sport will close their Group One campaign in Quito against already-eliminated Liga Deportiva Universitaria.