David Beckham will get the chance to win a record-equalling 108th cap for England - while Carlton Cole and Phil Jagielka could get their first after being named in the squad to face Spain in Seville next week.
Beckham needs one more appearance for the Three Lions to match Bobby Moore's best mark for an outfield player and coach Fabio Capello has decided the 33-year-old's form on loan at AC Milan warrants an instant recall after he was omitted for the win over Germany in Berlin last November.
The inclusion of Cole and Jagielka comes on the back of excellent performances at club level.
Milner is rewarded for his excellent performances in Aston Villa's rise to third spot in the Premier League table.
The former Leeds star has been capped an amazing 40 times for the under-21s but Capello recently hinted that Milner might be ready for the step up in standard.
And, even though Milner was selected last week for the side Stuart Pearce will take to Malaga for Tuesday's friendly with Ecuador, the midfielder will now move into the senior squad for the first time.
England squad to play Spain in a friendly international in Seville on Wednesday, February 11:
Goalkeepers: Green (West Ham), Hart (Man City), James (Portsmouth).
Defenders: Bridge (Man City), A Cole (Chelsea), R Ferdinand (Man Utd), Jagielka (Everton), G Johnson (Portsmouth), Terry (Chelsea), Upson (West Ham), L Young (Villa).
Midfielders: Beckham (LA Galaxy), Barry (Villa), Carrick (Man Utd), Downing (Middlesbrough), Lampard (Chelsea), Milner (Villa), Wright-Phillips (Man City), A Young (Villa).
Forwards: Agbonlahor (Villa), C Cole (West Ham), Crouch (Portsmouth), Heskey (Villa).