New W-League champions Queensland Roar have seven players in the 21-woman Australian squad chosen by coach Tom Sermanni to face Italy in two friendly fixtures.
Lana Harch, the W-League player of the year, and captain Kate McShea head the Roar contingent in the Matildas for the matches in Parramatta on January 31 and Canberra on February 7.
Sydney's Danielle Brogan, Canberra's Ellie Brush and Emily van Egmond, 15-year-old daughter of Newcastle Jets boss Gary van Egmond, all received their first call-ups to the national squad.
"The squad has a good balance of experienced, established players together with bright, young talent," Sermanni said on Thursday.
"Several players have stepped up to the mark and displayed good form throughout the W-League season, earning them a well deserved spot in the national team.
"It is this form and confidence which will serve the team well heading into these matches."
Matildas squad: Lydia Williams, Ellie Brush, Caitlin Munoz (Canberra United); Melissa Barbieri (Melbourne Victory); Amber Neilson, Jo Peters, Cheryl Salisbury, Emily van Egmond, Katie Gill (Newcastle Jets); Collette McCallum, Lisa de Vanna (Perth Glory); Lana Harch, Elise Kellond-Knight, Clare Polkinghorne, Karla Reuter, Kate McShea, Tameka Butt, Lauren Colthorpe (Queensland Roar); Danielle Brogan, Sarah Walsh, Heather Garriock (Sydney FC).