Blue Square North side Droylsden dumped League Two leaders Darlington out of the FA Cup with a 1-0 victory in their first-round replay.
Matthew Tipton (26) scored the winner with a strike from the edge of the area but the night was overshadowed by a serious-looking injury to Quakers striker Liam Hatch.
Hatch suffered a suspected broken neck during an aerial challenge and the game was delayed for more than half an hour before he could be taken to hospital by ambulance.
Elsewhere, Lincoln were stunned by Iyseden Christie's injury-time header which condemned them to a 2-1 home defeat at the hands of Blue Square Premier promotion hopefuls Kettering.
Lenell John-Lewis's header (53) had put the Imps ahead but Craig Westcarr (68) pegged them back before Christie's last-gasp winner.
Carlisle were thankful for a floodlight failure after 20 minutes which led to their tie against Blue Square Premier Grays being abandoned. The Cumbrians were trailing 1-0 at the time after Sam Sloma's 19th-minute goal.
Ridgeons Premier League side Leiston, the lowest-ranked side remaining in the competition, were knocked out after a 2-0 defeat at Fleetwood, who had Andy Bell (48) and Adam Warlow (79) to thank for their victory.
In the battle of two Blue Square Premier sides, Barrow hammered Eastbourne 4-0 thanks to goals from Richard Brodie (45), Paul Brown (47), Matt Henney (57), and a long-range Carlos Logan free-kick (75).
League Two Dagenham defeated League One strugglers Hereford 2-1 after goals by Paul Benson (17) and Solomon Taiwo (33), with Kris Taylor's striker (34) proving only a consolation.
Rotherham's hopes of a repeat of their Carling Cup heroics were emphatically ended by League Two peers Aldershot who thrashed them 3-0 at Don Valley Stadium. Kirk Hudson (15), Marvin Morgan (55) and Kirk Hudson (81) scored for the visitors.
Southend stamped their authority on Blue Square North side Telford with a 2-0 victory courtesy of strikes from Simon Francis (74) James Walker (79).
Stockport thrashed Yeovil 5-0 at Edgeley Park after Danny Schofield had missed an early penalty for the visitors.
A header from Gary Dicker (six) gave the Hatters the lead before Aaron Brown was sent off for Town. Michael Rose (15), Matty McNeil (42), Anthony Pilkington (48) and James Vincent (90) completed the rout.
Nicky Forster (28) gave Brighton the lead at Hartlepool but they eventually succumbed to a 2-1 defeat following a Joel Porter penalty (66) and Gary Liddle's 70th-minute winner.
Lewis Montrose's 22nd-minute goal gave Cheltenham a 1-0 win at Oldham, while Oxford needed extra-time to beat 10-man Dorchester 3-1 at Haig Avenue.
Jamie Mudge (22) had put the hosts ahead but James Constable (78) equalised and Jamie Gleeson's sending-off in extra-time swung the match decisively in Oxford's favour.
Phil Trainer (109) and Yemi Odubade (120) took advantage of the numerical advantage to put the former League club through.
Adam Le Fondre's stunning hat-trick helped Rochdale come from two goals down to defeat Barnet 3-2 after extra-time.
John O'Flynn (10) and Albert Adomah (19) gave the Londoners an advantage but Le Fondre (56, 73 and 104) then ended their hopes.
Luton were taken to penalties against Altrincham after their 0-0 draw had been delayed by floodlight difficulties.
Despite having Sol Davies (67) sent off, the League Two side came through 4-2 with Paul McVeigh netting the final penalty.
The match between Grays Athletic and Carlisle was abandoned due to floodlight failure.