Holland trio Robin van Persie, Ibrahim Afellay and Khalid Boulahrouz all sat out training on Wednesday.
Striker Van Persie is nursing a slight foot injury, while playmaker Afellay and defender Boularouz both have minor ankle problems.
Midfielder Mark van Bommel joined the squad on Wednesday after playing in former Bayern Munich keeper Oliver Kahn's farewell game on Tuesday.
Oranje coach Bert van Marwijk will lead his team into his first competitive game in charge next Wednesday when they face Macedonia in a World Cup qualifier.
Before that, they face Australia in a friendly on Saturday.