Maximiliano Rodriguez will miss Argentina's friendly with Belarus on Wednesday due to injury.
Atletico Madrid doctors informed their Argentinian Football Association counterparts that the 27-year-old midfielder did not fly to Minsk because of 'a muscular injury', according to the AFA official website, www.afa.org.ar.
The full extent of the injury has not been publicised, with the Atletico Madrid website also describing the problem as 'a muscular injury'.
Coach Alfio Basile has not named a replacement so he will have to do with only 16 players in the fixture.
Ten days ago Martin Demichelis was withdrawn from the squad.
The Bayern Munich defender had picked up a right ankle injury during a pre-season friendly.
The match is a warm up to the resumption of the South American Zone World Cup qualifiers, with Argentina second in the standings.
They meet leaders Paraguay on September 6.
Squad: Roberto Abbondanzieri (Getafe), Juan Pablo Carrizo (Lazio); Gabriel Heinze (Real Madrid), Gonzalo Rodriguez (Villarreal), Javier Zanetti, Nicolas Burdisso (both Internazionale), Fabricio Coloccini (Newcastle); Esteban Cambiasso (Internazionale), Pablo Ledesma (Catania), Luis Gonzalez (Porto), Jonas Gutierrez (Newcastle); German Denis (Napoli), Lisandro Lopez (Porto), Carlos Tevez (Manchester United), Fernando Cavenaghi (Bordeaux), Mariano Gonzalez (Porto).