Manchester City's miserable start to the season plumbed new depths as Chesterfield dumped them out of the Carling Cup with a 2-1 victory at Saltergate.
City were joined by Premiership rivals Fulham in exiting the competition after defeat by lower-league opposition tonight.
Greece international Georgios Samaras gave Stuart Pearce's full-strength side a 40th-minute lead, following up after Bernardo Corradi headed against a post.
But Caleb Folan's 47th-minute header quickly dampened City spirits early in the second half and the visitors' miserable season continued when Derek Niven put the League One side in front courtesy of a long-range volley.
Hapless Fulham were powerless to overcome a two-goal deficit at Craven Cottage as they lost 2-1 to Coca-Cola League Two leaders Wycombe.
The lively Jermaine Easter opened the scoring in the eight minute with a clinical finish before skipper Tommy Mooney doubled the score from the spot.
Heidar Helguson, who wasted Fulham's best first-half opportunity, gave his side a lifetime with a headed goal early in the second half.
New Aston Villa chairman Randy Lerner watched from the stands at Glanford Park as his side beat Scunthorpe 2-1.
Juan Pablo Angel scored the opener three minutes before half-time, following up his own penalty after Joe Murphy had saved. Luke Moore earned the sport-kick after getting hauled down by Richard Hinds.
Angel grabbed his second with an emphatic header from Aaron Hughes' cross, before effervescent Iron striker Billy Sharp headed home from a corner to pull one back.
Middlesbrough, winners in 2004, joined City and Fulham in crashing out after an abject 1-0 defeat at home to Notts County.
Congolese striker Tcham N'Toya fired Notts County into a shock half-time lead. The 22-year-old pounced on a 26th-minute error by full-back Tony McMahon to give the Coca-Cola League Two side hope of pulling off an upset.
And Middlesbrough, with Mark Viduka, Robert Huth, Massimo Maccarone and Gaizka Mendieta in their side, were unable to force a comeback, to the frustration of their supporters.
Doncaster, who beat Manchester City and Aston Villa en route to the quarter-finals last season, beat Championship visitors Derby 8-7 on penalties after a 3-3 draw in open play.
Jonathan Forte and Brian Stock gave last season's League Cup giant-killers Doncaster a 2-0 lead against their at the break.
Forte got his second and Rovers' third before the hour mark but Derby launched a spirited comeback. Steve Howard registered after 74 minutes and veteran Darren Moore headed a second.
Arturo Lupoli completed a remarkable comeback at Belle Vue with an injury-time goal to take the game beyond the normal 90 minutes but spoilt his evening with a sending-off for two bookings.
Both sides scored their first seven attempts in the shoot-out before Wales international Lewin Nyatanga missed for Derby, allowing James O'Connor to score the winning kick past Lee Camp.