The Czech Republic have gambled on the returning Milan Baros in attack against Italy.
The striker has missed the first two games of the World Cup, but is recalled because Jan Koller is injured and Vratislav Lokvenc suspended.
Italy leave striker Luca Toni on the bench to play four midfielders, with Mauro Camoranesi and Gennaro Gattuso coming in.
USA coach Bruce Arena brings in Jimmy Conrad for the suspended Eddie Pope and brings back DaMarcus Beasley in midfield.
Ghana are without banned striker Asamoah Gyan.
Czech Republic: Cech, Grygera, Kovac, Rozehnal, Jankulovski, Plasil, Polak, Nedved, Poborsky, Rosicky, Baros. Subs: Blazek, Galasek, Heinz, Jarolim, Jiranek, Kinsky, Koller, Mares, Sionko, Stajner.
Italy: Buffon, Zambrotta, Cannavaro, Nesta, Grosso, Camoranesi, Pirlo, Perrotta, Gattuso, Totti, Gilardino. Subs: Amelia, Barone, Barzagli, Del Piero, Iaquinta, Inzaghi, Materazzi, Oddo, Peruzzi, Toni, Zaccardo.
Ghana: Kingston, Mensah, Pantsil, Shilla, Mohamed, Appiah, Essien, Dramani, Boateng, Amoah, Pimpong. Subs: Eric Addo, Otto Addo, Adjei, Ahmed, Kuffour, Owu, Pappoe, Quaye, Sarpei, Tachie-Mensah.
USA: Keller, Onyewu, Conrad, Cherundolo, Bocanegra, Dempsey, Reyna, Lewis, Beasley, Donovan, McBride. Subs: Albright, Berhalter, Ching, Convey, Hahnemann, Howard, Johnson, O'Brien, Olsen, Wolff.