Ryan Giggs and Craig Bellamy have both pulled out of Wales' friendly international against Trinidad and Tobago on Saturday in Austria.
Wales skipper Giggs damaged a toe in Sunday's 1-0 win over a Basque representative side in Bilbao, having scored the second-half winner to make it four goals in his last four appearances for his country.
Giggs did not seem to be in any discomfort after the match but has since been unable to train at Wales' team headquarters near San Sebastian.
After today's training session, which Giggs again sat out, boss John Toshack decided there was no point in taking him to Graz for the clash with the World Cup qualifiers.
Bellamy, who is getting married in a fortnight, has also been released from the squad, with Toshack planning to play several of his promising youngsters, including Bristol City's David Cotterill, from the start of the match.
The rest of the party leave the Basque region tomorrow to embark on a lengthy trip first to Madrid and then Vienna before a four-hour coach ride to Graz. Giggs and Bellamy are then expected to fly back to the UK.
Giggs' appearance in the Basque game in Athletic Bilbao's stadium was his first-ever away friendly for his country, and the opportunity of a second - in which he would have come up against his former Manchester United colleague Dwight Yorke - has now gone.
Five of the Wales Under-21 squad to face Estonia at Wrexham tonight will then fly out to Austria to supplement Toshack's squad, but it is not expected that there will be an additional player added to replace either Giggs and Bellamy.
The five to return to the senior squad are Craig Davies, Andy Crofts, Gareth Bale, Rhoys Wiggins and Arron Davies.