Daniele De Rossi will miss two weeks through injury after straining a thigh muscle during Roma's 1-1 draw with Inter Milan on Sunday night.
De Rossi, 29, underwent a scan on Tuesday which confirmed the news, which had forced him to leave the match at the Stadio Olimpico during the half-time break.
A statement published on the club's official website read: "Daniele De Rossi underwent diagnostic tests this morning after picking up an injury in Sunday's Serie A match against Inter. The exams revealed a minor hamstring injury. The player has already begun a rehabilitation program and is expected to recover in approximately two weeks."
That means the midfielder will be missing in Wednesday's Coppa Italia semi-final - also against the Nerazzurri - as well as Serie A fixtures involving Cagliari and Sampdoria but he could return in time for the visit of champions Juventus the following week.
De Rossi has been the subject of much transfer speculation this season after failing to fit coach Zdenek Zeman's tactical plan and as such has made just ten starts over the course of the season. The club maintain he is central to their plans and insist he will not be leaving.