Lazio have been charged over the alleged racist behaviour by their supporters during November's controversial Europa League game against Tottenham.
Lazio supporters were heard insulting Spurs' links with the Jewish community by chanting "Juden Tottenham'' at the away fans during the 0-0 draw at the Stadio Olimpico on November 22.
UEFA also announced Tottenham have been charged over separate crowd disturbances at the Group J game.
A statement from UEFA read: "UEFA has opened disciplinary proceedings against S.S. Lazio. Charges relate to the alleged racist behaviour of Lazio supporters during the Italian club's 0-0 UEFA Europa League Group J draw against Tottenham Hotspur FC in Rome on 22 November 2012.
"Proceedings will also be instigated against S.S. Lazio for throwing of missiles and/or fireworks by their supporters, incidents of a non-sporting nature, late team arrival at the stadium, and late handling of the team sheet.''
UEFA will hear both cases at a disciplinary meeting on January 24.
Spurs, the Football Association and Lazio all strongly condemned the fans who chanted the anti-Semitic songs during the game, which came 24 hours after a knife attack on Tottenham fans in the Italian capital.
In the reverse fixture at White Hart Lane back in October Lazio's fans were found guilty of monkey chanting towards three Tottenham players.
Information from the Press Association was used in this report.