Juventus say utility player Simone Pepe will be "out of action for a long time" after suffering a second tear to his left thigh muscle.
Pepe, 29, had returned to action only recently, making a 20-minute against Lazio last month following months on the sideline with the first injury.
But Juve confirmed that he had suffered a setback in a statement on their official website, which said: "Pepe will have to be out of action for a long time yet.
"Over the last few days, the player suffered a new muscular problem at the semi-membrane level of the left thigh.
"It is a I-II degree lesion in the area of the previous lesion, which had healed. To avoid further setbacks, he will have a period of complete rest and treatment, so will only return when his condition is completely healed."
The injury will be a blow to Juve coach Antonio Conte, because Pepe had been set to deputise for Kwadwo Asamoah when the Ghanaian heads to the African Nations Cup next month.