Di Matteo could be without as many as seven first-team players for Saturday's showdown at the Allianz Arena, and the Chelsea boss has admitted he may be forced to name youngsters on the bench.
Defenders Cahill and Luiz have not played since April after picking up hamstring injuries - Cahill in the Champions League semi-final against Barcelona and Luiz in the FA Cup win over Tottenham - but both players are set to return to training on Tuesday.
Luiz claimed on Monday that he was confident of being fit, but Di Matteo admitted Malouda, who was substituted as a precaution in Sunday's win over Blackburn, would be "touch and go".
He said: "Gary and David are going to try and train with us [on Tuesday]. Florent Malouda we still have to assess and it will be touch and go for him to be available for Saturday."