PSG coach Unai Emery has revealed the defending champions will be missing at least five regular first-team players at Niort.
Lyon goalkeeper Anthony Lopes has been suspended for one game for sparking a bizarre dispute with rivals St Etienne.
Ligue 1 leaders Monaco will face a trip to Marseille in the Coupe de France round of 16.
Unai Emery revealed his delight at his team's performance in the 4-0 thrashing of Stade Rennais in the Coupe de France round of 32 on Wednesday.
Julian Draxler scored twice as Paris Saint-Germain beat Rennes in the Coupe de France. Monaco are still alive after surviving against Chambly.
Dimitri Payet said he had "chills" when he came off the bench in extra-time to help Marseille edge through 2-1 against Lyon in the Coupe de France.