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Atleti were just eliminated from the Copa del Rey by Barcelona and face Celta Vigo on Sunday with their Liga title hopes on life support.
Real Madrid looked out of sorts in a three-man defence, but once a back four was reinstalled, Isco led Los Blancos to a 3-1 win at Osasuna.
The injury happened midway through the first half of the game at El Sadar, with Tano coming out the worst after a collision between he and Isco.
Cristiano Ronaldo's 24th minute near-post strike seemed to have set up Madrid for a straightforward win, but Osasuna's Sergio Leon had other plans.
Despite a win to remain top in La Liga, Herculez Gomez felt Real Madrid played down to the level of lowly Osasuna.
Real Madrid continued their march to the La Liga title, thanks to some sublime goals over Osasuna, in a wet and tough match.