Barcelona star Lionel Messi will be out for three weeks, while Jose Mourinho's Manchester United are to take on City in the EFL Cup fourth round.
Wednesday's La Liga encounter at the Anoeta Stadium saw the return of La Real midfielders Sergio Canales and Raul Navas.
Ayestaran was dismissed on Tuesday after the club's fourth defeat in as many La Liga games over the weekend.
Dermot Corrigan takes at a look at the five major talking points from Tuesday and Wednesday's La Liga action.
Diego Simeone praises the work rate of Antoine Griezmann on and off the ball, saying it motivates Atletico Madrid.
Luis Enrique recalls Barcelona's experience playing without Lionel Messi, and has confidence his team can persevere.