Real Madrid's Dani Carvajal and Isco are definitely out of next Tuesday's Champions League semifinal second leg at home to Bayern Munich, and could be ruled out for the rest of the season.
Spain playmaker Isco picked up a shoulder problem very early in the 2-1 first-leg victory in Munich, and was replaced at the break by youngster Marco Asensio who went on to net the winner.
Carvajal suffered a hamstring injury midway through the second half while marking Franck Ribery, with winger Lucas Vazquez dropping back to fill in for the remainder of the game.
Following tests on Friday morning the club have confirmed that both players are injured, without giving any likely recovery time. Isco's shoulder injury has been confirmed as a "left acromioclavicular sprain," while Carvajal has a " muscular injury in the left femoral biceps hamstring."
Both face a race against time to be fit for a potential Champions League final date with Liverpool or Roma on May 26.
Utility defender Nacho Fernandez is still sidelined with a hamstring problem, coach Zinedine Zidane confirmed at Friday's press conference -- meaning that either Vazquez or youngster Achraf Hakimi will take on the right-back role on Tuesday at the Bernabeu.
Asensio should keep his place in the team, but Karim Benzema and Gareth Bale will both be hoping to earn a recall, with the final selection depending on which tactical formation Zidane decides on.
Both Benzema and Bale are likely to start Saturday's La Liga game at home to Leganes, with Zidane expected to rest a number of key first teamers including Cristiano Ronaldo, Luka Modric and Raphael Varane.