Giorgio Chiellini will miss Juventus' first two games of the new Serie A season after an appeal against a three-match ban was rejected.
The defender was suspended for three games after he was found guilty of elbowing Roma's Miralem Pjanic through television evidence.
Chiellini sat out the Bianconeri's final match of the season against Cagliari, but his club appealed the decision.
On Thursday, the Italian FA's Federal Court of Justice upheld the full ban, confirming the first judgement after reviewing the video evidence of the two players' clash.
Consequently, the 29-year-old, who is currently representing Italy at the 2014 World Cup in Brazil, will have to watch his club start the defence of their third straight Serie A title from the stands.
He will be able to return to domestic action only in the second half of September, after Italy's first Euro 2016 qualifiers.
The 2014-15 Serie A season starts on the weekend of August 30/31.