Plymouth will be without first-choice central-defensive pairing Maxime Blanchard and Darren Purse for the visit of Aldershot in the Johnstone's Paint Trophy second round.
Blanchard will continue his spell on the sidelines after being ruled out of Saturday's 2-1 home defeat to AFC Wimbledon with concussion.
Purse picked up his fifth booking of the season against the Dons and will serve a one-match suspension.
Warren Feeney, Ross Jenkins and Onismor Bhasera will also be unavailable to manager Carl Fletcher.
Feeney and Jenkins are both struggling with groin injuries, while Bhasera has been called up to the Zimbabwe squad to face Angola in an Africa Cup of Nations qualifier.
Meanwhile, attacking pair Alex MacDonald and Guy Madjo have both ended their loan spells with the Pilgrims.
Peter Vincenti returns from suspension for Aldershot.
The midfielder has completed a three-match ban for his red card in last month's 1-0 defeat at Accrington and could well come back into manager Dean Holdsworth's startling line-up.
The Shots are in desperate need of a morale-boosting victory having fallen to a fourth successive defeat with a 1-0 home reverse to Chesterfield at the weekend, with their latest setback greeted by a chorus of boos from the home faithful.
Holdsworth kept faith with an unchanged line-up following the 4-3 loss at Torquay but changes are likely to be on the agenda at Home Park, with Vincenti coming into contention along with the likes of Adam Mekki, Josh Payne, Michael Rankine, Sonny Bradley and Kieron Cadogan.
Goalkeeper Ross Worner (back) is targeting a December return while midfielder Craig Stanley (broken leg) should be back in the new year.