Annan will miss top goalscorer Peter Weatherson against his former club Morton at Galabank due to a calf injury.
Steven Swinglehurst and Steven Black are both doubts and new recruit Kevin Nicoll will not feature as he is cup-tied.
Jack Breslin, who has been added on loan from Celtic, and former St Mirren striker Lewis Guy who signed on Monday are both in manager Jim Chapman's plans.
Chapman said: "Morton are favourites but Hamilton Accies were the favourites in the last round and we beat them. It would...