Serie A side Empoli crashed out of the Coppa Italia in their first game in this year's competition, losing 1-0 to Vicenza on Saturday.
Leonardo Gatto's 53rd-minute goal was the difference as second-tier Vicenza advanced to the fourth round.
Frosinone, newly promoted to Serie A this season, were also eliminated, losing to Spezia 6-5 on penalties after neither side could find a breakthrough in 120 minutes.
Palermo advanced with a 2-1 win over Serie B's Avellino.
Luca Rigoni opened the scoring for...